Elucidating mechanism cellular uptake removal protein
Furthermore, in comparison with the typically adopted gene delivery vector, lipofectamine 2000 (with gene transfection efficiency of 55±5% for p EGFP), the gene expression efficiency was improved significantly (70±3% for p EGFP).Due to the attractive optical and biological performances of these CPNPs, they may find broad applications for nucleic acid delivery in future applications of therapeutic genes or RNAs.As a consequence, exploring non-viral gene delivery materials has continuously received considerable attention because these materials can be structurally varied, are relatively safe, and have an ability to carry large and diverse genetic materials into cells.Until now, a number of studies involving non-viral gene delivery systems, such as cationic lipids having one or two hydrophobic tails, polycations, dendrimers and histones, were carried out [9-16].
This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). How to cite this article: Wei L, Zhang D, Zheng X, Zeng X, Zeng Y, Shi X, Su X, Xiao L. Available from of efficient non-viral gene delivery vector has aroused great attention in the past few decades.
Exploring intracellular movement may offer deep insight into the precise mechanism of non-viral gene delivery.
Therefore, there is a urgent need to develop sensitive and noninvasive methods that could be performed to overcome the challenges of gene therapy such as utilizing nano-dimensional materials to carry genes across cellular membrane barriers and exploiting unique optical (or magnetic) properties for noninvasive and spatiotemporal molecular imaging.
p EGFP (enhanced green fluorescent protein plasmid) and p Ds Red (red fluorescence protein plasmid) were gifted from Prof. Shuanglin Xiang (Hunan Normal University, college of life science).
Propylene glycol amine cationic lipids with different length of carbon tails (R12, R14, R16 and R18) were gifted from Prof. Youlin Zeng (Hunan Normal University, Chinese patent: #201110444269.5).